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Product online since: 14.09.2009 12:33:08
6,6' - TREHALOSE DIMYCOLATE unit 10mgCord Factor, source: Mycobacterium bovis , " Bacillus Calmette Guérin" (BCG)
| Item number: | TDM-10 | | Manufacturer: | Produced for BIOCLOT | | Delivery time: |  Available now |
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Excluding VAT (19%) (Base price: pro 1 mg) | | | |
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Used as an immunoadjuvant for the induction and non-specific stimulation of antibody production against unrelated antigens. This results in an inceased host resistance against infections and cancer, in the potentiation of vaccine immunogeneticity and in an incease of DNA vaccination efficacy. Description
- 6‘6-Trehalose dimycolate (TDM) exhibits strong immunomodulatory effects.
- Greatly enhances the efficacy of DNA vaccination, shown to facilitate single-shot prime-boost DNA vaccines (3).
- TDM from M. bovis has a lower granulomatogenic activity as compared to TDM from M. tuberculosis (5).
- Preinjection of TDM followed by antigen injection triggers elevated antibody production (2).
- Stimulates macrophages, enhances non-specific immune responses and stimulates the antigen-presenting ability (7, 10).
- Exhibits anti-tumor activity (2).
- Induces the production of both Th1-type and Th2-type cytokines (IFN-alpha, IFN-beta, IFN-gamma, IL-4, IL-6, IL-10, IL-12,TNF-alpha) as well as chemokines (MCP-1, MIP-1alpha, IL-8) (4, 6, 8, 9,11).
- Upregulates MHC class II expression on macrophages and causes expansion of Th1 cells (9).
- TDM is non-toxic on organisms and serves as protection against killing from macrophages. TDM becomes antigenic and highly toxic, when exposed to lipid surfaces.
Quality Control:
Purity: LC, Single Spot
Quality Control: MALDI-TOF
Endotoxin Content: LAL Test
Nonpyrogenic Storage Conditions:
Store at + 4 °C in the dark.
| Apperence: |
white powder |
| Solubility: |
clear colorless solution at 50 mg/ml in chloroform:methanol:water (90:10:1) |
| Specific rotation: |
+32 to +35 degrees c=1 in chloroform:methanol:water (90:10:1) | Purity by thin layer chromatography:minimum 99%
Recommended Usage: Use 100 µg to 1 mg TDM / ml when applied as an immunoadjuvant.
References:
1.Bekierkunst, A., I. S. Levij, et al. (1971), Science 174: 1240-1242.
2.Bekierkunst, A., E. Yarkoni, et al. (1971), Infect. Immun. 4: 256-263.
3.de Paula, L., S. C.L., et al. (2007), Genet Vacc Ther. 24(5): 2.
4.Emoto, M., Y. Emoto, et al. (1999), Eur. J. Immunol. 29: 650-659.
5.Fujita, Y., Y. Okamoto, et al. (2007), Microb. Pathog. 43(1): 10-21.
6.Guillemard, E., M. Geniteau-Legendre, et al. (1995), Antiviral Res. 28: 175-189.
7.Kierszenbaum, F., A. Zemian, et al. (1984), Infect. Immun. 43: 531-535.
8.Oswald, I. P., C. M. Dozois, et al. (1997), Infect. Immun. 65: 1793-1799.
9.Ryll, R., K. Watanabe, et al. (2001), Microbes Infect. 3: 611-619.
10.Yarkoni, E., L. P. Ruco, et al. (1979), Eur. J. Cancer 15: 1401-1407.
11.Ryll, R., Kumazawa, Y. et al. (2001), Microbiol. Immunol. 45 (12): 801-811 FOR RESEARCH USE ONLY. NOT FOR USE IN MEDICATION
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Samples are available on request |
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