Incubate 60 min at 22…25 °C
The Ovalbumin ELISA enables a fast and sensitive quantification of Ovalbumin in vaccines and other culture supernatants won by use of chicken eggs. Enzyme immunoassay for quantitative detection of Ovalbumin Principle of the ELISA The Ovalbumin-ELISA is a direct sandwich enzyme immunoassay using immobilized polyclonal anti-Ovalbumin-antibodies and anti Ovalbumin-HRPconjugateas detection system. Conjugate and samples are incubated simultaneously. Short incubation times, ready-to-use reagents, quantitative results (ng/ml). The Ovalbumin-ELISA enables the fast and sensitive quantification of Ovalbumin in vaccines and other culture supernatants won by use of chicken eggs. Test kit components
• 96 well microtitration plate
• 50 ml wash buffer, 10fold concentrated
• 50 ml sample diluent
• 6 x 1.0 ml Ovalbumin standards, ready to use
• 1.0 ml Ovalbumin control sample, ready to use
• 15 ml anti-Ovalbumin-HRP-conjugate, ready to use
• 15 ml TMB-/substrate solution, |
